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Code of Veterinary Practice For Poultry Processing Plant

tested will not contain this quantity leave the surplus
rinsing solution in the bottle.
b) Thoroughly wet, as far as possible, the whole surface
of the piece of equipment to be rinsed with the
rinse solution. Agitation of the solution by rotary or
other movement is necessary for dislodging micro-
organisms.
c) Return the rinse to the bottle. The solution should be
examined immediately, if this is not possible, any delay
shall be kept to a minimum and the sample shall be
cooled quickly to not more than 4ºC and maintained
between 0ºC and 4ºC until examined (preferably
within 36 hours).

3.2.1.6 Swab Technique
3.2.1.6.1 Accurate Count per Square Inch
a) Place cotton-tipped swabs in test-tubes
containing 10 ml of distilled water and plug
with cotton wool.
b) Sterilize in autoclave for 15 minutes at 15
pounds pressure at 120ºC.
c) Take sterile swabs in tubes to area to be
tested.
d) Remove sterile swabs from tube and at
the same time squeeze excess water
from swab against inside of tube. Replace
cotton plug on test-tube.
e) Using a sterile metal template, swab an area
of 20 cm on the surface to be examined.
2
It is important to keep the swabbing
technique uniform e.g. 20 times across
from side to side and 20 times up and
down, using same amount of pressure.
The swab is rotated simultaneously to
ensure that all parts of it make contact
with the surface under test.

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